Imbibition of wheat seeds: Application of image analysis**

*Corresponding author e-mail: [email protected] **This work was supported by the Internal Grant Agency of the Czech University of Life Sciences Prague, Project No. 20163001, (2016). A b s t r a c t . Image analysis is widely used for monitoring seeds during germination, and it is often the final phase of germination that is subjected to the greatest attention. However, the initial phase of germination (the so-called imbibition) also exhibits interesting behaviour. This work shows that image analysis has significant potential in the imbibition. Herein, a total of 120 seeds were analysed during germination tests, and information about seed size and shape was stored and analysed. It was found that the imbibition can be divided into two newly defined parts. The first one (‘abrupt imbibition’) consists mainly of the swelling of the seed embryo part and lasts approximately one hour. The second one, referred to as ‘main imbibition’, consists mainly of spatial expansion caused by imbibition in the other parts of the seed. The results presented are supported by the development of seed cross area and shape parameters, and by direct observation. K e y w o r d s : seed cross area, shape parameters, imbibition stages, embryo development